Weigh 5 g of the propolis sample that has been crushed, or 10 g of propolis ethanol extract (accurate to 0001 g in a 250 mL beaker, add an appropriate amount of 95 ethanol, and place it in an ultrasonic cleaner to make the sample Dissolve, let cool to room temperature, use this filter to pre-dry and weigh to constant weight filter paper, wash the beaker and filter paper 3 times with appropriate amount of ethanol 5 times. Then the residue and filter paper under 0 vacuum (vacuum degree: -0.096 MPa~0.1 MPa) hand-operated to constant weight, parallel experiment under phase conditions.
5.3.2.5 Calculate the content of ethanol extract according to formula
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